Chemical and Biological Inventory and SDS
Published:
Chemical and biological inventory with links to relevant SDS
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Resources
Transfection
Published:
Protocol of transient transfection
Proteome Desalting and Fractionation
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Preparation of TMT labeled samples for fractionation prior to mass spec analysis.
Rho0 Cell Generation
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Protocol for generation of mitochondrial null Rho0 cell lines
Chemical Proteomics
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General protocols for photolabeling and TMT-based quantification of probe targets using mass-spectrometry
Photolabeling and MS-based Target ID
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Detailed protocol for in situ photolabeling, click reaction, target enrichment, reduction/alkylation, reaction with tandem mass tags (TMT) and digestion.
OxPhos biochemical activity
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Protocol for assaying the activity of individual electron transport complexes in enriched mitochondria
Molecular Biology
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Common protocols for cloning, transfection and transduction into human cell lines
Mitochondrial_Isolation
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Quick protoocol for purification of mitochondria from cultured cells
Metabolomics
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Common protocols for metabolomic assays using mass spectrometry
High-Content Imaging
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Common protocols for image-based assays measuring cell cycle, replication, mitochondria and plasma membrane dynamics and metabolic flux
NGS
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Common protocols for WGS and RNA sequencing
DNA Electrophoresis
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Protocol for agarose gel electrophoresis of DNA
Cell Biology
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Common protocols for cell based assays