Proteome Desalting and Fractionation
Published:
Preparation of TMT labeled samples for fractionation prior to mass spec analysis.
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Resources
Chemical Proteomics
Published:
General protocols for photolabeling and TMT-based quantification of probe targets using mass-spectrometry
Photolabeling and MS-based Target ID
Published:
Detailed protocol for in situ photolabeling, click reaction, target enrichment, reduction/alkylation, reaction with tandem mass tags (TMT) and digestion.
Molecular Biology
Published:
Common protocols for cloning, transfection and transduction into human cell lines
Metabolomics
Published:
Common protocols for metabolomic assays using mass spectrometry
High-Content Imaging
Published:
Common protocols for image-based assays measuring cell cycle, replication, mitochondria and plasma membrane dynamics and metabolic flux
NGS
Published:
Common protocols for WGS and RNA sequencing
Cell Biology
Published:
Common protocols for cell based assays